Contrary to popular mythology, urine is not necessarily sterile. Because many of the bacterial species found in the human bladder cannot be grown in the lab, they’ve long gone unidentified. However, new sequencing techniques allow researchers to catalog bacteria without the necessity of first cultivating them. In this way, Linda Brubaker from Loyola University Chicago and her colleagues from that school, Indiana University and the University of North Texas have been able to find bacteria growing in the bladders of even asymptomatic women.
In the past, if you wanted to get a DNA sequence you’d need to get your hands on a large quantity of that DNA. If you were interested in a microbe, this meant growing a vial full of the little buggers. Nowadays, this is no longer necessary. Not only do tiny amounts of DNA suffice, but you don’t even need to separate out the DNA by species prior to sequencing. Thus, the researchers were able to throw all the DNA they collected into one bag and pull out any bacterial sequences that happened to be in there.
Women who were already scheduled for surgery were asked to give their urine for science. They provided a ‘clean-catch’ (the pee-in-a-cup method of urine collection) before surgery. While they were anesthetized, urine was also obtained both by catheter and by syringe directly out of the bladder.
Bacteria were found using all three urine collection methods, even in women with no symptoms of urinary tract infection. In only two out of twenty-three women did syringe aspiration of urine directly from the bladder not yield bacteria.
No comments:
Post a Comment